ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (6): 1011-1017.doi: 10.11843/j.issn.0366-6964.2015.06.018

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Studies on the Pathology of the Peste des Petits Ruminants of Sheep

WANG Guang-xiang1,2,JIA Ning1* ,FANG Mei1,SUN Bao1   

  1.  (1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)
  • Received:2014-09-19 Online:2015-06-23 Published:2015-06-23

Abstract:

This experiment was conducted to explore the pathological changes of the peste des petits ruminants (PPR) of sheep.On the basis of clinical diagnosis,then the six doubtful sheep of the PPR were definite diagnosed by RT-PCR detection technology.The pathological changes were observed by macroscopy and light microscope and special staining technique.The results showed that the specific N gene fragment of 350 bp of peste des petits ruminants virus (PPRV) were amplified and the PPRV was definite diagnosed as the pathogen of the six sick sheep.The anatomopathological changes showed there were a lot of yellow mucopurulent nasal discharge in nasal cavities of the sheep and there were also many secretions around the eyes.The ulcers of oral cavity mucous membrane were frequently observed.The sheep had pneumonia of different degree and there were numerous yellowish-white lesions from pinpoint-shaped to millet-shaped on the surface of the livers and spleen.The lymphoglandulae of the sheep were obviously swelling and showed serous lymphadenitis and hemorrhagic lymphadenitis.Hemorrhagic stripes,plaques and ulcers were observed on intestinal mucosa and especially on small intestinal mucosa.The histopathological changes indicated that a great number of multiple nuclear giant cells or syncytial cells were observed in lung,bronchus,liver,spleen and lymphoglandulae.The multiple nuclear giant cells or syncytial cells showed decentralized and concentrated distribution in the tissues and organs and there were eosinophil viral inclusions in their cytoplasm.Lung lesions of bronchointerstitial pneumonia were seen obviously in the sheep.There were numerous macrophages,lymphocytes in alveoli and interstitial tissue of the lungs.The parenchymal cells of the liver,kidney and spleen showed widely degeneration and necrosis.A great number of macrophages,plasma cells and lymphocytes were seen in the interstitial tissue of the liver,kidney and spleen.Those results confirmed the proliferation of multiple nuclear giant cells or syncytial cells and bronchointerstitial pneumonia were importantly histopathological changes of the peste des petits ruminants of sheep.

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